datp incorporation Search Results


32 p]datp incorporation kit  (Promega)
90
Promega 32 p]datp incorporation kit
Elevated MIP-1α expression in MCMV-infected livers. ( A ) Liver RNA was isolated from uninfected and infected mice. Reverse transcriptase PCR cDNA products were generated and amplified using <t>oligonucleotide</t> primers specific to MIP-1α or the housekeeping gene, GAPDH. Amplified DNA was detected by Southern blot analysis and hybridization with specific internal oligonucleotide probes as described in Materials and Methods. Samples were from C57BL/6-MIP-1α −/− mice infected with MCMV for 2 d (lane 1 ), or C57BL/6 mice that were uninfected (lane 2 ) or infected with MCMV for 1, 2, 3, and 5 d (respectively, lanes 3–6 ). ( B ) Livers were harvested from day 2 MCMV-infected C57BL/6 mice, and frozen tissue sections were prepared and used for immunohistochemical staining of MIP-1α protein as described in Materials and Methods. The MIP-1α protein is identified by dark blue precipitates, and tissues are counterstained with methyl green. Panel photograph was taken at ×31.25. Inset photograph, showing higher power of an inflammatory focus, was taken at ×125. Bar, 100 μm.
32 P]Datp Incorporation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/32 p]datp incorporation kit/product/Promega
Average 90 stars, based on 1 article reviews
32 p]datp incorporation kit - by Bioz Stars, 2026-03
90/100 stars
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random primed [32p]-datp incorporation prime-a-gene labeling kit  (Promega)
90
Promega random primed [32p]-datp incorporation prime-a-gene labeling kit
Elevated MIP-1α expression in MCMV-infected livers. ( A ) Liver RNA was isolated from uninfected and infected mice. Reverse transcriptase PCR cDNA products were generated and amplified using <t>oligonucleotide</t> primers specific to MIP-1α or the housekeeping gene, GAPDH. Amplified DNA was detected by Southern blot analysis and hybridization with specific internal oligonucleotide probes as described in Materials and Methods. Samples were from C57BL/6-MIP-1α −/− mice infected with MCMV for 2 d (lane 1 ), or C57BL/6 mice that were uninfected (lane 2 ) or infected with MCMV for 1, 2, 3, and 5 d (respectively, lanes 3–6 ). ( B ) Livers were harvested from day 2 MCMV-infected C57BL/6 mice, and frozen tissue sections were prepared and used for immunohistochemical staining of MIP-1α protein as described in Materials and Methods. The MIP-1α protein is identified by dark blue precipitates, and tissues are counterstained with methyl green. Panel photograph was taken at ×31.25. Inset photograph, showing higher power of an inflammatory focus, was taken at ×125. Bar, 100 μm.
Random Primed [32p] Datp Incorporation Prime A Gene Labeling Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primed [32p]-datp incorporation prime-a-gene labeling kit/product/Promega
Average 90 stars, based on 1 article reviews
random primed [32p]-datp incorporation prime-a-gene labeling kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Elevated MIP-1α expression in MCMV-infected livers. ( A ) Liver RNA was isolated from uninfected and infected mice. Reverse transcriptase PCR cDNA products were generated and amplified using oligonucleotide primers specific to MIP-1α or the housekeeping gene, GAPDH. Amplified DNA was detected by Southern blot analysis and hybridization with specific internal oligonucleotide probes as described in Materials and Methods. Samples were from C57BL/6-MIP-1α −/− mice infected with MCMV for 2 d (lane 1 ), or C57BL/6 mice that were uninfected (lane 2 ) or infected with MCMV for 1, 2, 3, and 5 d (respectively, lanes 3–6 ). ( B ) Livers were harvested from day 2 MCMV-infected C57BL/6 mice, and frozen tissue sections were prepared and used for immunohistochemical staining of MIP-1α protein as described in Materials and Methods. The MIP-1α protein is identified by dark blue precipitates, and tissues are counterstained with methyl green. Panel photograph was taken at ×31.25. Inset photograph, showing higher power of an inflammatory focus, was taken at ×125. Bar, 100 μm.

Journal: The Journal of Experimental Medicine

Article Title: Early Murine Cytomegalovirus (MCMV) Infection Induces Liver Natural Killer (NK) Cell Inflammation and Protection Through Macrophage Inflammatory Protein 1α (MIP-1α)–dependent Pathways

doi:

Figure Lengend Snippet: Elevated MIP-1α expression in MCMV-infected livers. ( A ) Liver RNA was isolated from uninfected and infected mice. Reverse transcriptase PCR cDNA products were generated and amplified using oligonucleotide primers specific to MIP-1α or the housekeeping gene, GAPDH. Amplified DNA was detected by Southern blot analysis and hybridization with specific internal oligonucleotide probes as described in Materials and Methods. Samples were from C57BL/6-MIP-1α −/− mice infected with MCMV for 2 d (lane 1 ), or C57BL/6 mice that were uninfected (lane 2 ) or infected with MCMV for 1, 2, 3, and 5 d (respectively, lanes 3–6 ). ( B ) Livers were harvested from day 2 MCMV-infected C57BL/6 mice, and frozen tissue sections were prepared and used for immunohistochemical staining of MIP-1α protein as described in Materials and Methods. The MIP-1α protein is identified by dark blue precipitates, and tissues are counterstained with methyl green. Panel photograph was taken at ×31.25. Inset photograph, showing higher power of an inflammatory focus, was taken at ×125. Bar, 100 μm.

Article Snippet: Probes were labeled by incorporation of [ 32 P]dATP at the 5′-end of each oligonucleotide using the kit as described by manufacturer ( Promega , Madison, WI).

Techniques: Expressing, Infection, Isolation, Reverse Transcription, Generated, Amplification, Southern Blot, Hybridization, Immunohistochemical staining, Staining